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KMID : 1177720080020020011
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Journal of Alternatives to Animal Experiments
2008 Volume.2 No. 2 p.11 ~ p.14
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Spontaneous biliary carcinogenesis in zebrafish
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Seok Seung-Hyeok
Park Jae-Hak
Kim Dong-Jae
Na Yi-Rang
Baek Min-Won
Han Ju-Hee
Koh Ae-Sun
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Abstract
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Zebrafish is proposed as an ideal vertebrate cancer model. Because zebrafish is easy to maintain and breed, and her fecundity is high enough (200～300 eggs per fish). Powerful genetics and cellular biology tools are also available in zebrafish. Even, histologically many organs are very similar with humans. We have raised over 1,000 fish for the transgenesis study through injections of various genes in a closed recirculating water system. In the end of the study, before euthanasia, we biopsied the negative control fish at 24 months of age. The histological analysis of one case of zebrafish tumorous change revealed biliary carcinoma, based upon the H&E staining. The local invasion, fibrosis, and increased mitotic index were observed in laboratory maintained wild type zebrafish. This is the first documentation of the spontaneous biliary carcinoma in wild type zebrafish in Korea.
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KEYWORD
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Zebrafish, biliary carcinoma, cancer
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